Introduction: quality of life is a multidimensional concept that important and various factors such as physical and psychological effect on it. Moreover, the Job stress is one of the most important factors in reduced productivity in organizations. The present study was performed to determine quality of life and its relationship with Job stress among nursing staff in hospitals of sari, in 2015. Method: This descriptive- analytical study was performed on 940 people in among nursing staff in hospitals of Sari, in 2015. The samples were selected by census sampling method. Data were gathered using particular questionnaires of quality of life (SF-36) and HSE (Health and Safety Executive) Job stress. Collected data were analyzed by ANOVA, t-test, Pearson's correlation and Linear Regression using SPSS software (V18). Results: The majority of samples with frequency of 62.2% had a moderate quality of life. Also The Mean±SD of Job stress was 115.02±20.94 that represents a great stress in nurses. Also a significant correlation observed between general aspects physical health and mental health with all aspects of job stress except Control and support of authorities (P<0.05). In addition, sex, work and body mass index with quality of life disorders and also between history, marital status and working system with a job stress was a significant relationship (P<0.05). Conclusion: Findings showed that increases a job stress has a negative effect on some aspects of quality of life. So, nursing managers by aware of this result, with proper communication and support them in order to reduce the physical requirements, psychological pressure and stuff participation in decision-making in order to reduce job stress and following that increase job quality and improve the quality of life, act.